LncRNA HOXA11-AS Promotes Proliferation and Cisplatin Resistance of Oral Squamous Cell Carcinoma by Suppression of miR-214-3p Expression
Figure 4
Inhibition of miR-214-3p suppressed the effect of HOMA11-AS knockdown on proliferation, CDDP chemosensitivity, and apoptosis in CDDP-resistant OSCC cells. (a) mRNA expression level of miR-214-3p in tumor tissues and corresponding noncancerous tissues of 31 OSCC patients (p=0.012). (b) mRNA expression level of miR-214-3p in OSCC cell lines (TSCCA and CAL-27) and their corresponding CDDP-resistant cell lines (TSCCA-CDDP and CAL-27-CDDP) (p=0.004; p=0.007). TSCCA-CDDP and CAL-27-CDDP cells were cotransfected with si-HOMA11-AS and anti-miR-214-3p or their negative controls. ((c)–(f)) Cell proliferation and IC50 value of CDDP were determined by CCK8 assay kit ((c) p=0.013; p=0.027; (d) p=0.009; p=0.022; (e) p=0.026; p=0.031; (f) p=0.013; p=0.038). ((g) and (h)) Apoptosis was evaluated by TUNEL assay ((g) p=0.028; p=0.032; (h) p=0.029; p=0.027). ((i) and (j)) Caspase 3 activities were detected using assay kit ((i) p=0.012; p=0.015; (j) p=0.019; p=0.016). #P < 0.05.