Optimization and Standardization of Thermal Treatment as a Plasma Prefractionation Method for Proteomic Analysis

Figure 2

Consistency and reproducibility of thermal treatment as demonstrated by the 2-DE analysis (50 μg/gel). Untreated plasma and TS soluble fraction after thermal treatment (n=3 individual samples) were separated by 2-DE using the IPG strip pH 4-7 (linear) for the first dimension and 12.5% SDS-PAGE for the second dimension. Protein spots were visualized by the blue silver CBB-G250 staining [4] and then detected by ImageMaster 2D-Platinum program. The arrow indicated a protein spot that is exclusively presented in subject 3.