PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway

Figure 3

MiR-214 downregulation inhibits osteoclast differentiation. (a) TRAP staining in osteoclasts expressing miR-214 mimic or inhibitor or NC after M-CSF and RANKL induction. (b) Relative miR-214 expression in osteoclasts transfected with miR-214 mimic or inhibitor or NC was detected by qRT-PCR. (c) qRT-PCR of CTSK, NFATc1, and ACP5 in cells expressing miR-214 mimic or inhibitor or NC after M-CSF and RANKL induction. (d) Western blotting of CTSK, NFATc1, and ACP5 in cells expressing miR-214 mimic or inhibitor or NC after M-CSF and RANKL induction.

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