Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?
Figure 2
rv3632 gene presence and transcription. (a) Amplification of hsp65 gene from gDNA isolated from the following: (1) M. tuberculosis H37Rv; (2) M. tuberculosis H37Ra; (3) M. bovis; (4) M. bovis BCG; (5) M. smegmatis; (6) PC: positive PCR control; NC: negative PCR control. MWM: 50 bp molecular weight marker (b) Amplification of rv3632 gene from gDNA isolated from the following: (1) M. tuberculosis H37Rv; (2) M. tuberculosis H37Ra; (3) M. bovis; (4) M. bovis BCG; (5) M. smegmatis; (6) PC: positive PCR control; NC: negative PCR control. (c) hsp65 gene amplification of cDNA from the following: (1) M. tuberculosis H37Rv plus synthesis; (2) M. tuberculosis H37Rv minus synthesis; (3) M. tuberculosis H37Ra plus synthesis; (4) M. tuberculosis H37Ra minus synthesis; (5) M. bovis plus synthesis; (6) M. bovis minus synthesis; (7) M. bovis BCG plus synthesis; (8) M. bovis BCG minus synthesis; (9) M. smegmatis plus synthesis; (10) M. smegmatis minus synthesis; (11) PC: positive PCR control (M. tuberculosis H37Rv gDNA); NC: negative PCR control; MWM: 50 bp molecular weight marker. (d) rv3632 gene amplification of cDNA from the following: (1) M. tuberculosis H37Rv; (2) M. tuberculosis H37Ra; (3) M. bovis; (4) M. bovis BCG; (5) M. smegmatis; (6) PC: positive PCR control; NC: negative PCR control. MWM: 50 bp molecular weight marker.