Specific Binding Peptides from Rv3632: A Strategy for Blocking Mycobacterium tuberculosis Entry to Target Cells?

Figure 3

Detecting Rv3632 on mycobacterial surface. (a) Western blot recognition of Rv3632 in M. tuberculosis H37Rv. Lane 1: pre-immune sera. Lane 2: post-third immunisation of polymer 40057 against Mtb H37Rv lysate. Lane 3: recognition of hyper-immune control sera (obtained by inoculating Mtb H37Rv sonicate). Complete Mtb H37Rv protein lysate was used as antigen. (b) IEM assay localisation of Rv3632 on Mtb H37Rv envelop. Electron density was observed on bacillus surface due to colloidal gold-labelled antibody, indicated by white arrows.

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