Usnea Acid as Multidrug Resistance (MDR) Reversing Agent against Human Chronic Myelogenous Leukemia K562/ADR Cells via an ROS Dependent Apoptosis

Figure 2

UA Plus Adr Induced ROS Generation, Apoptosis, and Cell Cycle Arrest in K562/Adr Cells. Cells were treated with UA(4 μM), Adr UA(4 μM), and UA plus Adr (4 μM) for 48 hr before examination; medium with same concentration of DMSO was used as control. Annexin V-positive cells were analyzed by flow cytometry (a, d). Cell cycle arresting was observed by PI-staining DNA flow cytometric analysis (b, e). ROS generation was detected by confocal microscopy and flow cytometry (c, f). Columns: values are expressed as mean ± SD.

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