LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression

Figure 4

LncRNA-TWIST1-knockdown increased the TWIST1 expression and inhibited the osteogenic differentiation of HPDLSCs. (a–c) The mRNA levels of TWIST1, β-catenin, and cyclin D in HPDLSCs infected by lncRNA-TWIST1-knockdown lentivirus. (d) The mRNA expression of osteogenic genes in HPDLSCs infected by the lncRNA-TWIST1-knockdown lentivirus. (e) Alizarin red and ALP staining were used to detect osteogenic differentiation. (f) Quantitative measurement of Alizarin red staining. (g) ALP activity assay. (h) Schematic diagram depicting possible mechanisms involved in the effect of LncRNA-TWIST1 (P< 0.05, P< 0.01). Mean ± SEM.

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