Identification of Key Transcription Factors AP-1 and AP-1-Dependent miRNAs Forming a Co-Regulatory Network Controlling PTEN in Liver Ischemia/Reperfusion Injury

Figure 5

PTEN was transcriptionally activated by AP-1 while inhibited by AP-1-dependent miRNAs in cellular hypoxia/reoxygenation model. (a) Results of AP-1 reporter gene luciferase assay. (b) Results of PTEN promoter luciferase reporter assay. (c) Results of the luciferase reporter assay of 3’UTR of PTEN. Cellular hypoxia/reoxygenation model was used in luciferase reporter assays. (d) miR-212-3p, miR-22-3p, miR-29a-3p, miR-92a-3p expression levels in mice liver I/R sample were examined by qPCR. SP600125 is a Jun N-terminal kinase (JNK) inhibitor.  < 0.05,  < 0.01,  < 0.001,  < 0.0001.

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