HIF-1-miR-219-SMC4 Regulatory Pathway Promoting Proliferation and Migration of HCC under Hypoxic Condition

Figure 3

SMC4 was transcriptionally regulated by HIF-1 under hypoxic condition. (a) The result of coexpression analysis between SMC4 and HIF-1a was shown. r = 0.4201, value = 2.691e − 17. (b) Huh7 cells were cotransfected with HRE-wild-vector or HRE-wild and HRE-mutant-vector or HRE-mutant, and the luciferase activities were examined. The firefly luciferase activity of each sample was normalized to the Renilla luciferase activity. n = 3, . (c) Expressions of HIF-1a, SMC4, CDK2, CDK4, E-cadherin, and Vimentin were detected by western blot under hypoxia or normoxia or hypoxia + HIF-1a siRNA.

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