HIF-1-miR-219-SMC4 Regulatory Pathway Promoting Proliferation and Migration of HCC under Hypoxic Condition

Figure 4

Hypoxia increases the expression of SMC4 through inhibiting the expression of miR-219. (a) mRNA level of SMC4 was detected under hypoxic or normoxic condition in HCC cells. n = 3, . (b) The expression of miR-219 was detected under hypoxia or normoxia or hypoxia + HIF-1a siRNA in HCC cells. n = 3, , .(c) Expression of SMC4 was detected under treatment of miR-219 mimics, inhibitor, or negative control, and β-actin was used for internal control. (d) CCK-8 was used to detect cell viability of HCC cells with miR-219 mimics, inhibitor, or negative control under hypoxic condition. The value of 72 h was statistically analyzed. n = 7, . (e) Transwell assay was used to detect cell migration ability of HCC cells with miR-219 mimics, inhibitor, or negative control under hypoxic condition. n = 3, .

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