Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma

Figure 1

Effects of paeoniflorin on proliferation, migration and invasion, and actin cytoskeleton arrangement in T98 and U251 cells. (a-b) cells were incubated with the indicated concentrations of paeoniflorin for 24 hours before CCK-8 assay. (c, d) cells were incubated with 0μM, 5μM or 10μM proliferation after the wounds were scratched. Then representative images of wound healing were acquired after 0 or 24 hours. (e, f) The transwell invasion assay. Cells were treated with the indicated dose of paeoniflorin for 24 hours. Then representative picture was taken. The stained cells were counted. Each represents at least three independent experiments. (g, h) Confocal sections of T98 and U251 cells stained with FITC-phalloidin after indicated concentration paeoniflorin treatment and the quantitative analysis of the number of actin filaments. All tests were performed in triplicate and presented as mean ± standard error. P<0.01, P<0.001, compared with control (0 μM).

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