Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma

Figure 6

SU11274 suppressed HGF-mediated RhoA/ROCK signaling activity in T98G and U251. T98G and U251 cells were treated with 20 ng/ml of HGF for 15 minutes in present or absent of preincubation with SU11274 (5μmol/l) for 6 hours, protein level of c-Met and p-c-Met (a-d), GTP-RhoA activity (e-h), protein expression of ROCK1, Limk1, and p-Limk1 (i-l) were detected by western blotting. Control: no treatment; HGF: 20ng/ml HGF; SU: c-Met inhibitor SU11274 (5μmol/l); HGF+SU: 20ng/ml HGF combined with 5μmol/l SU11274 (5μmol/l) treatment. All tests were performed in triplicate and presented as mean ± standard error. P < 0.05, compared with control group. P < 0.05, compared with HGF group.

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