Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma

Figure 7

Over-expression of c-Met reduced effects of paeoniflorin on cell migration, invasion,n and actin cytoskeleton rearrangement. T98G and U251 were transfected vector plasmid or c-Met overexpression plasmid and treatment with or without 10μM paeoniflorin; then the cell migration (a-d) and invasion (e-h) and actin cytoskeleton rearrangement and number (i-j) were evaluated. Control: vector plasmid; PF: 10μM paeoniflorin +vector plasmid; ex-c-Met: c-Met overexpressed plasmid; PF+ex-c-Met: c-Met overexpressed plasmid +10μM paeoniflorin. All tests were performed in triplicate and presented as mean ± standard error. P < 0.05, compared with control group. P < 0.05, compared with PF group.

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