Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma

Figure 8

Overexpression of c-Met reduced effects of paeoniflorin on c-Met/RhoA/Limk1 signaling. T98G and U251 were transfected vector plasmid or c-Met overexpression plasmid and then treatment was with or without 10μM paeoniflorin for 24h. The activity of GTP-RhoA (a-h) and the protein expression of ROCK1, Limk1, and p-Limk1 (i-l) were examined. Control: vector plasmid; PF: 10μM paeoniflorin +vector plasmid; ex-c-Met: c-Met overexpressed plasmid; PF+ex-c-Met: c-Met overexpressed plasmid +10μM paeoniflorin. All tests were performed in triplicate and presented as mean ± standard error. P < 0.05, compared with control group. P < 0.05, compared with PF group.

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