The Effect of MicroRNA-331-3p on Preadipocytes Proliferation and Differentiation and Fatty Acid Accumulation in Laiwu Pigs

Figure 3

miR-331-3p expression during preadipocyte differentiation and confirmation of its target genes. (a) Level of miR-331-3p in preadipocytes differentiation process. (b) miR-331-3p influenced PPARγ expression in preadipocytes differentiation process. (c) The seed region of DLST and SLC25A1 CCAGGG mutated to CTGATCG using overlapping PCR. (d) Two pmirGLO vector constructs, containing either the DLST-3′-UTR (or SLC25A1-3′-UTR) or the DLST-3′-UTR (or SLC25A1-3′-UTR) with a mutation in the miR-331-3p seed region, were transfected into PK-15 cells either alone or in combination with NC or miR-331-3p mimic. Renilla luciferase activity was normalized to firefly luciferase. Data represent means ± SD (; ). Different lowercase letters denoted significant difference () and different capital letters represent very significant differences ().

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