The Effect of MicroRNA-331-3p on Preadipocytes Proliferation and Differentiation and Fatty Acid Accumulation in Laiwu Pigs

Figure 4

miR-331-3p can also promote the synthesis of fatty acids by regulating the target gene DLST to promote acetyl-CoA translocation. (a) The expression of DLST and SLC25A1 measured by qRT-PCR. (b) DLST and SLC25A1 protein were detected by Western blotting. (c) DLST and SLC25A1 protein expression were quantified. (d) DLST coding dihydrolipoamide succinyltransferase and SLC25A1 coding mitochondrial citrate transporter played a critical role in citrate pyruvate cycle. (e) Some genes coding enzymes of citrate pyruvate cycle were measured by qRT-PCR. (f) Images of cells stained with Oil Red O after miR-331-3p mimics, miR-331-3p inhibitor, and miR-NC transfection; (g) Quantification of fatty acid of adipocyte. Data represent means ± SD (; ).

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