The Effect of MicroRNA-331-3p on Preadipocytes Proliferation and Differentiation and Fatty Acid Accumulation in Laiwu Pigs

Table 2

Primer sequences used for quantitative real-time polymerase chain reaction and gene cloning.

Gene	GenBank accession no.	Primer sequence (5′–3′)
DLST	NC_010449.5	AGCCCCAAAAGCAGAACC
		GGGCAGCAGTGGGTTTTA
SLC25A1	NC_010456.5	TGACCAGACCTCTTCCAA
		AACGAAGAAGCGGATGGC
GAPDH	NC_010447.5	AGGTCGGAGTGAACGGATTTG
		ACCATGTAGTGGAGGTCAATGAAG
CDK2	NC_010447.5	AAGATGGACGGAGCTTGTTATCGC
		CTGGCTTGGTCACATCCTGGAAG
CDK3	NC_010454.4	TCATCCACCGAGACCTGAAGCC
		AGACATCCACAGCCGTCGAGTAG
CDK4	NC_010447.5	TGAGATGGAGGAGTCTGGAGCAC
		CTCGGAAGGCAGAGATTCGCTTG
Cyclin B	NC_010458.4	GACTGGCTAGTGCAGGTTCAGATG
		ATGGCAGTGACACCAACCAGTTG
CDKN1A	NC_010449.5	CGAGAGCGATGGAACTTCGACTTC
		TCCACATGGTCCTCCTGAGACG
PPARγ	NC_010455.5	AGGACTACCAAAGTGCCATCAAA
		GAGGCTTTATCCCCACAGACAC
CS	NC_010447.5	ACACTCAACTCAGGACGGGT
		TTCCAGGAGGACATTGGGCA
ACLY	NC_010454.4	CTTGATCCGCAAACCTGCCT
		CCGTCACCATCAGGCACATC
DLD	NC_010451.4	GGCGCAGTGCACATTGACTA
		ACCATGCCATCTGTGTCAGC
SDHA	NC_010458.4	CCTGGAGTTCGTGCAGTTCC
		AACCTCTCACCCTGGCTGTT
FH	NC_010452.4	TGGTGCCCAGACTGTGAGAT
		AGCTGCACGCTTCAAGATCC
ME1	NC_010443.5	TTGCAGCCCTTCGAATCACC
		AGGTGTGCAATCCCTAAGGC
PC	NC_010444.4	AGAACGAGATCCCAGGAGGC
		GACTCAGCCCATTCTGCACC
MDH1	NC_010445.4	CATGCCAAGAAGGGATGGCA
		CCTTGGGAATGGATGGAGCC