Research Article

Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli

Table 2

Summary of BglA optimization in different media (LB, TB, and M9NG) with IPTG and lactose as inducers.

Expression inducerGrowth mediumMax OD600BglA % in TCPBglA mg/ml

IPTGLB3.69500.6
TB5.49700.7
M9NG3.47400.5

LactoseLB6.56501.5
TB16.46702.0
M9NG15.18702.2

Note. TCP: total cell protein. Concentration determined through relative densitometry.