Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli

Table 3

Summary of BglA recovery and activity at different stages of purification.

Purification stages	Total protein (mg/ml)	Percentage recovery (%)^a	Percentage recovery (%)^b	Total activity (U)	Specific activity (U/mg)	Fold purification
Crude cell extract	29.85	100	100	18704	627	1
Soluble fraction	17.84	60	70	14250	799	1.27
Treatment of soluble fraction at 65°C	15.57	52	30	13601	874	1.39
Ni-chromatography	3.8	13	15	11807	3107	4.96

^aPercentage recovery (%) determined by Bradford assay [19]. ^bPercentage recovery (%) determined by densitometry after SDS-PAGE.