Hepatitis B Virus X Protein-Induced Serine Protease Inhibitor Kazal Type 1 Is Associated with the Progression of HBV-Related Diseases
Figure 2
The effect of HBV on SPINK1 expression in cells. (a) The expression level of SPINK1 mRNA in HepG2.2.15 and HepG2 cells detected using RT-qPCR. (b) Expression of the SPINK1 protein in HepG2.2.15 and HepG2 cells was detected using western blotting. (c) Changes in luciferase activity at 48 hours after cotransfection of HepG2 cells with 0.2 μg of SPINK1 promoter SPINK1-Luc and 0.6 μg of HBV1.3 or its control plasmid pBlue-ks; the experiment was repeated three times. (d) SPINK1 mRNA expression was detected using RT-qPCR at 48 hours after transfection of HepG2 cells with 4 μg of HBV1.3 or its control plasmid pBlue-ks. (e) SPINK1 protein expression detected using western blotting at 48 hours after transfection of HepG2 cells with 4 μg of HBV1.3 or its control plasmid pBlue-ks. indicates P<0.05.