Hepatitis B Virus X Protein-Induced Serine Protease Inhibitor Kazal Type 1 Is Associated with the Progression of HBV-Related Diseases

Figure 3

The effect of HBx on SPINK1 expression. (a) Changes in luciferase activity at 48 hours after cotransfection of HepG2 cells with 0.6 μg of the eukaryotic expression plasmid containing each gene of the HBV genome (pCMV-S, pCMV-E, pCMV-C, pCMV-X, and pCMV-P) and 0.2 μg of the SPINK1 gene promoter SPINK1-Luc or its control plasmid pCMV-tag2B; the experiment was repeated three times. (b) SPINK1 mRNA expression detected using RT-qPCR at 48 hours after transfection of HepG2 cells with 4 μg of pCMV-X or its control plasmid pCMV-tag2B. (c) SPINK1 protein expression detected using western blotting at 48 hours after transfection of HepG2 cells with 4 μg of pCMV-X or its control plasmid pCMV-tag2B. indicates P<0.05.

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