Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Figure 2

Effects of YNB on odonto/osteogenic differentiation of SCAPs. (a) Representative images of ARS and calcium nodules under the inverted microscope (scale bars = 200 μm). (b) Quantitative analyses of calcium nodules. Values are means ± SD, n=3, P<0.01. (c) Immunofluorescent staining of RUNX2 in the control group and YNB group. Scale bar = 200 μm. (d) Immunofluorescent staining of COL-I in the control group and YNB group. Scale bar = 200 μm. (e, f) mRNA expressions of OCN, OPN, OSX, RUNX2, ALP, COL-I, and DSP in different groups by real-time RT-PCR at day 3 and day 7. Values are described as means ±SD, n=3. , P<0.01; , P<0.05. (g, j) The expression levels of the odonto/osteogenic proteins in the control group and YNB group at day 3 and day 7, respectively. (h, i) Grayscale analysis of the odonto/osteogenic markers at day 3. n=3. P<0.05, P<0.01. (k, l) Grayscale analysis of the odonto/osteogenic markers at day 7. n=3. P<0.05, P<0.01.