BioMed Research International

Research Article

Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA

Table 1

List of organism used for analytical specificity test.


Organism	Source	No. tested ()	Results in duplex qPCR

Aspergillus fumigatus	USM, Malaysia	1	Negative
Bacillus subtilis	USM, Malaysia	1	Negative
Burkholderia cepacia	USM, Malaysia	6	Negative
Burkholderia pseudomallei	USM, Malaysia	20	Positive
Burkholderia thailandensis	USM, Malaysia	1	Negative
Campylobacter jejuni	USM, Malaysia	1	Negative
Candida albicans	USM, Malaysia	1	Negative
Citrobacter freundii	USM, Malaysia	1	Negative
Entamoeba histolytica	UNAM, Mexico	1	Negative
Enterococcus faecalis	USM, Malaysia	1	Negative
Klebsiella pneumoniae	USM, Malaysia	1	Negative
Leptospira biflexa serovar Patoc	IMR, Malaysia	1	Positive
Leptospira biflexa serovar Patoc	UPM, Malaysia	1	Positive
Leptospira borgpetersenii Celledoni	IMR, Malaysia	1	Positive
Leptospira borgpetersenii serovar Ballum	UPM, Malaysia	1	Positive
Leptospira fainei serovar Hurtsbridge	IMR, Malaysia	1	Positive
Leptospira fainei serovar Hurtsbridge	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Australis	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Autumnalis	IMR, Malaysia	1	Positive
Leptospira interrogans serovar Bataviae	IMR, Malaysia	1	Positive
Leptospira interrogans serovar Bataviae	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Canicola	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Copenhageni	IMR, Malaysia	1	Positive
Leptospira interrogans serovar Hebdomadis	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Icterohaemorrhagiae RGA	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Javanica	IMR, Malaysia	1	Positive
Leptospira interrogans serovar Pomona	IMR, Malaysia	1	Positive
Leptospira interrogans serovar Pomona	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Pyrogenes	IMR, Malaysia	1	Positive
Leptospira interrogans serovar Pyrogenes	UPM, Malaysia	1	Positive
Leptospira interrogans serovar Tarassovi	IMR, Malaysia	1	Positive
Leptospira licerasiae serovar Varillal	IMR, Malaysia	1	Positive
Leptospira meyeri serovar Semaranga	IMR, Malaysia	1	Positive
Leptospira wolffii	IMR, Malaysia	1	Positive
Plasmodium falciparum	MKA Kota Bharu	5	Negative
Plasmodium knowlesi	MKA Kota Bharu	5	Negative
Plasmodium vivax	MKA Kota Bharu	5	Negative
Proteus mirabilis	USM, Malaysia	1	Negative
Proteus vulgaris	USM, Malaysia	1	Negative
Salmonella Paratyphi A (ATCC 9150)	ATCC, USA	1	Negative
Salmonella Paratyphi B (ATCC BAA 1250)	ATCC, USA	1	Negative
Salmonella Paratyphi C (ATCC 9068)	ATCC, USA	1	Negative
Salmonella Typhi (ATCC 7251)	ATCC, USA	1	Negative
Salmonella Typhimurium (ATCC 14028)	ATCC, USA	1	Negative
Staphylococcus aureus	USM, Malaysia	1	Negative
Staphylococcus saprophyticus	USM, Malaysia	1	Negative