Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA

Table 3

Analytical sensitivity of the duplex qPCR assays.

Copies number	Amount (pg)	Duplex qPCR for B. pseudomallei			Copies number	Amount (pg)	Duplex qPCR for Leptospira spp.
		Mean Cq	SD	CV (%)			Mean Cq	SD	CV (%)
2560000	20000	18.41	0.05	0.26	4000000	20000	19.3	0.17	0.86
256000	2000	21.75	0.31	1.43	400000	2000	23.09	0.16	0.67
25600	200	25.34	0.12	0.46	40000	200	27.23	0.04	0.16
2560	20	28.97	0.13	0.44	4000	20	30.67	0.32	1.05
256	2	32.8	0.14	0.44	400	2	34.63	0.08	0.24
25.6	0.2	36.96	1.06	2.88	40	0.2	36.34	0.5	1.37
2.56	0.02	-	-	-	4	0.02	38.59	1.49	3.87
0.256	0.002	-	-	-	0.4	0.002	-	-	-