Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of<i> Burkholderia pseudomallei</i> and<i> Leptospira</i> spp. DNA

<table class="fixed-width table-group" id="tab4"><tr><td><table class="table"><colgroup><col style="width:6.29em"/><col style="width:7.19em"/><col style="width:7.46em"/></colgroup><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left" rowspan="2">Parameter</td><td class="align_center" colspan="2">Target</td></tr><tr class="thead"><td class="align_center"><i>Leptospira</i> spp.</td><td class="align_center"><i>B. pseudomallei</i></td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">Slope</td><td class="align_center">-3.2776</td><td class="align_center">-3.7006</td></tr><tr><td class="align_left">Efficiency</td><td class="align_center">101.9%</td><td class="align_center">86.3%</td></tr><tr><td class="align_left">Linearity, R<sup>2</sup></td><td class="align_center">0.9837</td><td class="align_center">0.9987</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>PCR efficiency and linearity of the duplex qPCR assays.</div>

BioMed Research International

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Table 4

Table 4: Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of<i> Burkholderia pseudomallei</i> and<i> Leptospira</i> spp. DNA 

Table 4 | Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of Burkholderia pseudomallei and Leptospira spp. DNA 