APC-Cdh1 Inhibits the Proliferation and Activation of Oligodendrocyte Precursor Cells after Mechanical Stretch Injury
Figure 1
Mechanical stretching-induced decreases in OPCs proliferation and enhanced expression of cellular Cdh1. Purified OPCs were seeded into BioFlex six-well plates and connected to a stretching system to conduct mechanical injury of the cells, which were statically cultured for 48 h after being loaded according to the treatment grouping. (a) MTT detection of cell viability after mechanical injury. The OD value of the injured group gradually decreased with the extension of stretching time and was lower as compared with the control group. (b, c) Flow cytometric analysis of the effects of mechanical injury on OPCs cell cycle distribution. The proportion of injury group cells in S phase was significantly decreased as compared the control group, and it had a tendency of decrease with time. (d, e) Effects of mechanical stretch injury on protein expression of Cdh1 in OPCs. Western blot analysis showed increased protein expression of Cdh1 in the injury group as compared the control group. Additionally, expression of Cdh1 increased with progression of mechanical stretching time. Relative expression levels were determined by densitometry and normalized to β-actin expression as an internal reference. (f) Effects of mechanical stretch injury on Cdh1 mRNA expression in OPCs. Real-time fluorescent quantitative PCR analysis suggested that, compared with the control group, the Cdh1 mRNA expression in the injury group was significantly increased, and it had a tendency of increase with the extension of mechanical stretch time. N = 3; P < 0.05 versus Control; #P < 0.05 versus Stretch at 2 hours; and P < 0.05 versus Stretch at 6 hours.