APC-Cdh1 Inhibits the Proliferation and Activation of Oligodendrocyte Precursor Cells after Mechanical Stretch Injury
Identification of the effectiveness of adenoviral vector-mediated interference of Cdh1 RNA expression. The adenovirus was diluted, and the Cdh1-shRNA adenovirus construct and the empty adenovirus vector were separately added to the cells at an MOI = 60, following which, cells were harvested 72 hours after transduction. (a, b) Effects of Cdh1-shRNA adenovirus transduction on Cdh1 expression and its downstream protein substrates Skp2 and Id2 in OPCs. Western blot analysis demonstrated results showed that the protein expression of Cdh1 in OPCs was significantly decreased as compared the empty vector in the control group; additionally, the protein expression of Skp2 and Id2 was significantly increased after Cdh1-shRNA adenovirus transduction. (c) Cdh1 expression and its downstream substrates Skp2 and Id2 mRNA after Cdh1-shRNA adenovirus transduction. Real-time PCR analysis showed that Cdh1 mRNA expression in OPCs was significantly decreased as compared transduction with the empty vector in the control group; further, Skp2 and Id2 mRNA expression was significantly increased after Cdh1-shRNA adenovirus transduction. N = 3; P < 0.05 versus Ad-Control.