BioMed Research International / 2019 / Article / Fig 3

Research Article

APC-Cdh1 Inhibits the Proliferation and Activation of Oligodendrocyte Precursor Cells after Mechanical Stretch Injury

Figure 3

Adenoviral-mediated Cdh1 RNA interference promoted OPCs proliferation and activation following mechanical stretching injury. After OPCs were respectively infected for 72 hours with Cdh1-shRNA adenovirus and the empty adenoviral vector, the mechanical stretching injury was applied for 12 hours. After completing the loading, static culture was continued for another 48 hours. (a, b) Cdh1 protein expression levels in the Control, Stretch, Ad-Cdh1-Stretch, and Ad-Control-Stretch groups are shown. Western blot analysis showed that, compared with the Ad-Control-Stretch group, adenoviral-mediated Cdh1 RNA interference significantly downregulated Cdh1 protein expression in OPCs after mechanical injury. (c) MTT assay of cell viability. Compared with Ad-Control-Stretch group, the OD value of the Ad-Control-Stretch group was significantly increased but was not at the level seen in the normal control group. (d, e) Flow cytometry of cell cycle distribution. The proportion of S phase cells in the Ad-Cdh1-Stretch group was significantly higher than that seen in the Ad-Control-Stretch group. N = 3; P < 0.05 versus Control; #P < 0.05 versus Stretch; and P < 0.05 versus Ad-Cdh1-Stretch.

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