BioMed Research International / 2019 / Article / Fig 4

Research Article

APC-Cdh1 Inhibits the Proliferation and Activation of Oligodendrocyte Precursor Cells after Mechanical Stretch Injury

Figure 4

Changes in the expression of the APC-Cdh1 downstream substrates Skp2 and Id2 in OPCs after mechanical injury. (a-c) Effects of mechanical stretching on protein expression of Skp2 and Id2 in OPCs. After stretching the cells by group treatment, OPCs were cultured for 48 hours, and the total protein was assayed by Western blotting. The protein expression levels of Skp2 and Id2 after mechanical stretching were lower than those of the control group and decreased time-dependently. N = 3; P < 0.05 versus Control; #P < 0.05 versus Stretch-2-hour; and P < 0.05 versus Stretch-6-hour. (d-f) The effects of dampened Cdh1-dependent protein expression of Skp2 and Id2 in OPCs after mechanical injury. Following transduction of OPCs for 72 hours with Cdh1-shRNA adenovirus and the empty adenoviral vector, mechanical stretch injury was performed for 12 hours. Following completion of the loading, static culture was continued for an additional 48 hours. Western blotting demonstrated that protein expression of Skp2 and Id2 in the Ad-Cdh1-Stretch group was significantly increased as compared to that in the Stretch group and the Ad-Control-Stretch group. N = 3; P < 0.05 versus Control; #P < 0.05 versus Stretch; and P < 0.05 versus Ad-Cdh1-Stretch.

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