Follistatin-Like 1 Attenuates Ischemia/Reperfusion Injury in Cardiomyocytes via Regulation of Autophagy
Effect of FSTL1 pretreatment on autophagy induction in H9C2 cells exposed to hypoxia/ischemia. (a) Western blot shows the effects of FSTL1 pretreatment on p62, beclin-1, and LC3 expression. (b) Quantitation of P62 and beclin-1 expression was performed using three independent experiments. P < 0.05 vs. the control group, #P < 0.05 vs. the hypoxia/ischemia group. (c) Quantitation of LC3 was performed using three independent experiments. P < 0.05 vs. the control group, #P < 0.05 vs. the hypoxia/ischemia group. (d) H9C2 cells under different conditions were cotransfected with RFP-LC3 and GFP-LC3, and viewed under a confocal microscope. The yellow dots represent autophagosomes. The red dots represent autolysosomes. (e) The level of autolysosomes and autophagosomes was analyzed. The red represents autolysosomes. The yellow represents autophagosomes. P < 0.05 vs. the control group, #P < 0.05 vs. the hypoxia/ischemia group. All experiments were repeated at least three times.
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