Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate

<table class="figure-group"><tr class="fig-image" id="a"><td><object data="https://static.hindawi.com/articles/bmri/volume-2019/9540702/figures/9540702.fig.002a.svgz" name="9540702.fig.002a" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(a) </b>FBS coated plate</td></tr><tr class="fig-image" id="b"><td><object data="https://static.hindawi.com/articles/bmri/volume-2019/9540702/figures/9540702.fig.002b.svgz" name="9540702.fig.002b" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(b) </b>Uncoated plate</td></tr></table>

<div>Flow cytometry analysis of GFP gene transfer efficiency and expression in K562 cells transduced by increasing MOI of lentiviral vector particles after 48 h. (a) shows K562 cells which are cultured in FBS coated plate and percentage of GFP expression. (b) shows K562 cells which are cultured in the uncoated plate and percentage of GFP expression. Vertical axes present fluorescent emission (FL-1) and horizontal axes present side scatter (SSC). Values within the gated area show the percentage of GFP gene expression in gated cells. The gate in control nontransduced samples was set to 1 %.</div>

BioMed Research International

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Figure 2

Figure 2: Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate 