Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate

Figure 3

K562 cells transduction efficiency for different MOIs grown in FBS coated plate and uncoated plate (bar: mean ± SEM). To examine the effect of coating FBS on lentiviral infection, K562 was cultured in FBS coating plate which promote cells attachment to plate surface and compared with K562 cells cultured in uncoated plate cells. K562 cells which are grown in coated plate showed significant increase in the transduction percentage compared with cells in uncoated plate group. Asterisk indicates significant difference between two groups by t-test (P < 0.05).P<0.05; P<0.01.