A Pathway Analysis Based on Genome-Wide DNA Methylation of Chinese Patients with Graves’ Orbitopathy

Figure 1

The flowchart of the study. Firstly, the pathway analyses were performed to extract the significant pathways based on the DNA methylation profile of the differentially methylated regions (DMRs). Secondly, for each significant pathway, the Methylation-based Inference of Regulatory Activity (MIRA) scores were calculated to infer the regulatory activity of genes involved in the pathway. Finally, the genes involved in the significant pathways were taken as the subsets, and random forest method was applied to these gene subsets to classify samples and to extract GO-related feature genes. DMRs: differentially methylated regions. MIRA: Methylation-based Inference of Regulatory Activity.