Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells

Figure 1

Western blot analysis of CCRs protein in PC12 cell lines. (a) Approximately 40–60 μg of total protein was resolved on a 10% SDS-PAGE gel and electroblotted onto nitrocellulose membranes. Membranes were probed with polyclonal anti-CCR1, polyclonal anti-CCR3, or monoclonal anti-CCR5. Representative blots are shown and arrows indicate the main band of the receptors with apparent MW 41 kDa for CCR1, 40 kDa for CCR3, and 46 kDa for CCR5. (b) The bands intensity was densitometrically analyzed and the results are expressed as % of the control PC12 cells obtained after normalization to endogenous β-actin level (± SD). P < 0.05 (n=7), C: control line, _2: PMCA2-reduced line, _3: PMCA3-reduced line.