A Bivalent Recombinant Mycobacterium bovis BCG Expressing the S1 Subunit of the Pertussis Toxin Induces a Polyfunctional CD4⁺ T Cell Immune Response

Figure 2

Bivalent expression of S1PT antigen in recombinant BCG. Western blot of rBCG strains expressing S1PT through distinct vectors. Protein extracts of wild-type BCG (BCG wt), rBCG-S1PT, rBCG-S1i and rBCG-S1+S1i were separated in soluble and insoluble fractions. S1PT antigen was detected using anti-S1PT previously generated in mice. The values in the center represent the molecular sizes in kilodaltons. Arrows indicate the presence of the two forms of S1PT expressed in rBCG.