Research Article

A Bivalent Recombinant Mycobacterium bovis BCG Expressing the S1 Subunit of the Pertussis Toxin Induces a Polyfunctional CD4+ T Cell Immune Response

Figure 3

In vitro stability of bivalent rBCG construct. rBCG-S1i and rBCG-S1+S1i were serially passaged in MB7H10 plates with and without kanamycin and the percentage of CFU retaining kanamycin resistance (Kan R+) was determined according to CFU quantification.