A Bivalent Recombinant Mycobacterium bovis BCG Expressing the S1 Subunit of the Pertussis Toxin Induces a Polyfunctional CD4⁺ T Cell Immune Response

Figure 4

Single integrative and bivalent rBCG vaccines induce inflammatory cytokines. Splenocytes from immunized mice were cultured for 48 h in presence of CFP or medium only (mock). Cytokine levels were measured by ELISA. Data are shown as the mean (±SEM) from n = 4-5 mice per group. Statistical analyses were performed by Student’s t test with a Mann-Whitney post-test. , ; ns = not significant.

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