[Retracted] Fbxw7 and Skp2 Regulate Stem Cell Switch between Quiescence and Mitotic Division in Lung Adenocarcinoma
Figure 1
Influence of 5-Fu on A549 cells. (a) Survival rates of A549 cells exposed to 5-Fu for 24 h, 48 h, or 72 h. Cell viability was detected by the MTT assay. (b) The protein expression of OCT4 in the N group, 0 h group, and 48 h group was determined by Western blotting assays. (c) The mRNA expression of OCT4 in the N group, 0 h group, and 48 h group was determined by qRT-PCR. (d) Effect of 5-Fu treatment on OCT4 protein expression was assessed by immunofluorescence microscopy. (e) The expression of β-catenin in A549 cells with or without 5-Fu treatment. (f) Effect of 5-Fu treatment and release of 5-Fu treatment for 48 h on A549 cell cycle distribution. Cell cycle distribution of G0/G1 and S phases was measured by flow cytometry. (g) The percentage of cells at different cycle phases was calculated. All the results are expressed as mean ± SD (n = 3, p < 0.05 significantly different from control group).