Integrated Bioinformatics Analysis of Master Regulators in Anaplastic Thyroid Carcinoma
Figure 7
Experimental validation of master regulators in different tissues and cell lines. (a) The mRNA expression levels of CREB3L1, E2F7, FOXM1, and NFYB in normal tissues (n=6) and ATC tissues (n=3). Results were presented as mean ± SD. (b) The expression levels of CREB3L1, E2F7, FOXM1, and NFYB in normal tissue and ATC tissue were detected by western blot. (c) Immunohistochemistry staining of CREB3L1, E2F7, FOXM1, and NFYB in normal tissue and ATC tissue. (d) The mRNA expression levels of CREB3L1, E2F7, FOXM1, and NFYB in Nthy-ori 3-1 and three ATC cell lines. Results were presented as mean ± SD, n=3. < 0.05, < 0.01, and < 0.001. (e) The expression levels of CREB3L1, E2F7, FOXM1, and NFYB in Nthy-ori 3-1 and three ATC cell lines were detected by western blot. (f) Heatmap of the candidate markers in microarray as retrieved from the proteomics data in the literature. Grey block indicated candidates were not significantly changed in microarray dataset. NT, normal thyroid tissues; ATC, anaplastic thyroid carcinoma.