[Retracted] Exosomes Derived from Human Bone Marrow Mesenchymal Stem Cells Stimulated by Deferoxamine Accelerate Cutaneous Wound Healing by Promoting Angiogenesis
Figure 6
Effects of DFO-Exos with miR-126 knockdown on angiogenesis of endothelial cells. Levels of miR-126 in exosomes from BMSCs treated with DFO + PBS (DFO-Exos), DFO + negative inhibitor control (NCI-DFO-Exos), or DFO + miR-126 inhibitor (126I-DFO-Exos) were analyzed by qRT-PCR. P < 0.001 versus DFO-Exos, ### P < 0.001 versus NCI-DFO-Exos group. (b) Western blotting analysis of PTEN, p-AKT, and AKT expression in HUVECs among three groups. (c) Left panel, representative images of transwell assay, scale bar = 200 μm; right panel, quantitative analysis of the assay. P < 0.001 versus DFO-Exos, ### P < 0.001 versus NCI-DFO-Exos group. (d) Left panel, representative images of tube formation assay from three groups, scale bar = 200 μm; right panel, quantitative analysis of the assay. P < 0.001 versus DFO-Exos, ### P < 0.001 versus NCI-DFO-Exos group. (e) Schematic diagram depicts the enhanced proangiogenic effects of DFO-Exos on wound healing. Followed by subcutaneous administration of DFO-Exos, Exos, or PBS, higher rate of wound closure was observed in rats treated with DFO-Exos due to the improvement of angiogenesis.