P4HB, a Novel Hypoxia Target Gene Related to Gastric Cancer Invasion and Metastasis
HIF-1α and P4HB are upregulated in human GC cell lines, and HIF-1α regulates the expression of P4HB in GC cells. (a, c) HIF-1α and P4HB protein expression in GC cell lines (SGC-7901 and BGC-823) and the human normal gastric epithelial cell line (GSE-1) by western blot. β-actin was used as a loading control; n = 3, : P < 0.01. (b, d) HIF-1α and P4HB mRNA expression in SGC-7901, BGC-823, and GSE-1 by RT-PCR; n = 3, : P < 0.01. (e) (A) HIF-1α was overexpressed in GC tissue. (B) HIF-1α was weakly expressed in adjacent tissues. (C) P4HB was overexpressed in GC tissue. (D) P4HB was weakly expressed in adjacent tissues. In all figures, 200× magnification was used. (f, g) The score of HIF-1α and P4HB in 40 GC tissues was of statistical significance compared to that of the corresponding adjacent nontumor specimens by IHC. Data are presented as the mean± SD (n= 40).