Emergence of New Delhi Metallo-Beta-Lactamase (NDM) and Klebsiella pneumoniae Carbapenemase (KPC) Production by Escherichia coli and Klebsiella pneumoniae in Southern Vietnam and Appropriate Methods of Detection: A Cross-Sectional Study

Table 1

Primers and probes used in this study.

Oligonucleotide	Nucleotide sequence, 5′–3′	Origin	Volume of reaction (nM)	Reference
Primers and probes for real-time PCR
NDM-F Primer	GAC CGC CCA GAT CCT CAA	IDT	300
NDM-R Primer	CGC GAC CGG CAG GTT	IDT	300
NDM-probe	HEX-TG GAT CAA GCA GGA GAT-ZEN/IBFQ	IDT	200
KPC-F Primer	GGC CGC CGT GCA ATA C	IDT	500
KPC-R Primer	GCC GCC CAA CTC CTT CA	IDT	500	[32]
KPC- probe	6FAM-TG ATA ACG CCG CCG CCA ATT TGT-ZEN/IBFQ	IDT	200
16S rRNA-F Primer	TGG AGC ATG TGG TTT AAT TCG A	IDT	200
16S rRNA-R Primer	TGC GGG ACT TAA CCC AAC A	IDT	200
16S rRNA-probe	Cy5-CA CGA GCT GAC GAC ARC CAT GCA-IBRQ	IDT	100
Primers for DNA sequencing
NDM-F-seq	AGT CGC TTC CAA CGG TTT	IDT	300	This study
NDM-R-seq	CAT TGG CAT AAG TCG CAA TCC	IDT	300
KPC-F-seq	GGT CAC CCA TCT CGG AAA	IDT	500
KPC-R-seq	GGG ATG GCG GAG TTC AG	IDT	500