Uncoupling Protein 2 Drives Myocardial Dysfunction in Murine Models of Septic Shock
Figure 1
UCP2 is activated in murine septic hearts. (a) Representative images (upper panel) and results of echocardiographic (lower panel) assessment 12 h after intraperitoneal injection of PBS or LPS in mice (n = 5 for each group). (b) Survival of WT mice intraperitoneally injected with PBS or LPS, monitored every 3 h for a 60-h period (n = 8 for PBS, n = 12 for LPS). (c) Western blot detection of UCP2 protein levels in cardiac tissues 12 h after intraperitoneal injection of PBS or LPS in WT mice (n = 5 for each group). (d) Real-time PCR analysis of UCP2 protein levels in cardiac tissues 12 h after intraperitoneal injection of PBS or LPS in WT mice (n = 3 for each group). Data were presented as means ± SEM. P<0.001 vs. the indicated group. IVSd, interventricular septum thickness at end-diastole; IVSs, interventricular septal thickness at end-systole; LVIDd, left ventricular internal diastolic diameter; LVIDs, left ventricular internal systolic diameter; LVPWd, left ventricular diastolic posterior wall thickness; LVPWs, left ventricular systolic posterior wall thickness.