Uncoupling Protein 2 Drives Myocardial Dysfunction in Murine Models of Septic Shock
Figure 2
UCP2 knockdown resists septic cardiac dysfunction. (a) Western blot analysis of knockdown efficiency of AAV9-shUCP2 4 weeks after delivery of virus. n = 4 samples per group. P<0.01 vs. the indicated group. (b) Representative images and results of echocardiographic assessment 12 h after intraperitoneal injection of PBS or LPS in AAV9-NC or AAV9-shUCP2 mice (n = 5 for each group). Data were presented as means ± SEM. P < 0.05, P < 0.01, and P < 0.001 vs. the indicated group. (c) Survival of AAV9-NC (n = 8 for PBS, n = 12 for LPS) or AAV9-shUCP2 (n = 8 for PBS, n = 12 for LPS) mice intraperitoneally injected with PBS or LPS, monitored every 3 h for a 60-h period. Data were presented as means ± SEM. P < 0.001 vs. the AAV-NC+PBS group; ###P < 0.001 vs. the AAV-NC+LPS group.