Uncoupling Protein 2 Drives Myocardial Dysfunction in Murine Models of Septic Shock

Figure 4

UCP2 knockdown activated AMPK-ULK1 signaling pathway and enhanced LPS-induced changes of cardiac autophagy. Representative immunoblots (upper panel) and quantitative analysis (lower panel) of AMPK, ULK1, light chain-3 (LC3), P62, and beclin 1 in LPS-challenged AAV9-NC or AAV9-shUCP2 mice, using GAPDH as a loading control in heart tissue lysates. Data were presented as means ± SEM. P < 0.05, P < 0.01, and P < 0.001 vs. the corresponding group.