IgE, IgG1, and IgG4 Reactivity to Dermatophagoides pteronyssinus Glycosylated Extract in Allergic Patients

Figure 1

Fractionation profile of D. pteronyssinus crude extract (Dpt, 5 mg of total protein) on a ConA-Sepharose affinity column. ConA-unbound and ConA-bound fractions were eluted with binding buffer and 0.1 M methyl α-D-mannopyranoside (), respectively.