IgE, IgG1, and IgG4 Reactivity to Dermatophagoides pteronyssinus Glycosylated Extract in Allergic Patients

Figure 6

SDS-PAGE profile (a) of the D. pteronyssinus crude extract (Dpt, lane 1) and ConA-unbound (lane 2) and ConA-bound (lane 3) fractions on visualization by silver staining. Three representative sera from DP+ group and one from NA group used on Western Blotting for IgE (b), IgG1 (c), and IgG4 (d) are exposed. Protein standard molecular weights are indicated in kDa.

(a) SDS-PAGE

(b) IgE

(c) IgG1

(d) IgG4