IgE, IgG1, and IgG4 Reactivity to Dermatophagoides pteronyssinus Glycosylated Extract in Allergic Patients

Table 1

Total protein, carbohydrate, Der p 1, and Der p 2 contents in the D. pteronyssinus (Dpt) crude extract and ConA-unbound and ConA-bound fractions. Results are expressed in absolute values (µg/ml) or allergen content in relation to the total protein content (%).

Sample	Protein (µg/ml)	Carbohydrate (µg/ml)	Protein/Carbohydrate ratio	Der p 1 (µg/ml/%)	Der p 2 (µg/ml/%)
Dpt	5,000	2,500	2.0	293/5.9	308/6.1
ConA-unbound	1,300	700	1.8	135/10.3	87/6.7
ConA-bound	500	200	2.5	25/5.0	4.2/0.8

Determined by Lowry method. Determined by Dubois method. Determined by ELISA.