Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MS/MS and Its Application in Pharmacokinetics and Bioavailability

Table 3

Precision, accuracy, extraction efficiency, and matrix effect of rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine, hirsutine, and hirsuteine in mouse blood ().

Compound	Concentration (ng/mL)	Accuracy (%)		Precision RSD (%)		Matrix; Effect	Recovery
		Intraday	Interday	Intraday	Interday
Rhynchophylline	1	105.2	108.4	12.0	14.1	106.7	68.7
	4	101.6	93.4	9.1	10.1	108.4	67.6
	90	97.1	106.6	10.6	7.4	103.6	64.6
	900	103.0	98.6	4.9	8.7	102.0	72.0
Isorhynchophylline	1	108.8	109.0	14.0	11.1	94.1	71.0
	4	97.6	90.6	11.6	6.8	103.3	83.5
	90	98.8	109.8	4.7	7.5	100.8	69.5
	900	102.9	96.3	5.1	2.5	95.6	79.5
Corynoxeine	1	97.8	90.7	11.3	13.3	106.1	75.9
	4	100.7	97.6	12.5	8.9	105.6	69.6
	90	99.0	104.6	5.1	12.2	107.4	73.9
	900	95.5	96.3	6.8	4.5	106.6	81.3
Isocorynoxeine	1	86.5	110.4	9.5	12.1	108.4	79.2
	4	97.0	92.9	11.4	11.7	105.9	86.8
	90	107.4	93.1	9.0	12.3	106.1	81.4
	900	101.3	100.2	7.2	6.4	104.0	80.9
Hirsutine	1	107.6	92.7	11.9	14.1	103.9	78.8
	4	97.1	97.0	6.1	13.5	102.4	72.6
	90	107.0	96.0	5.9	10.5	109.4	70.3
	900	104.3	97.8	5.0	9.0	107.8	76.4
Hirsuteine	1	98.7	109.0	13.6	12.0	103.6	73.3
	4	108.1	97.3	7.9	8.5	105.1	64.4
	90	102.1	94.7	8.6	10.2	105.6	76.1
	900	100.2	105.9	10.0	3.2	108.2	65.0