Transient Receptor Potential Channel Canonical Type 3 Deficiency Antagonizes Myofibroblast Transdifferentiation In Vivo

Figure 3

Effects of TRPC3 on ROS production and mitochondrial function in human fibroblasts. Mitochondrial oxygen consumption was measured in human fibroblasts treated with TGFβ1 (10 ng/mL) for 24 h via Oxygraph-2k high-resolution respirometry. (a) The test protocol is shown. (b) The values are expressed in pmol/s per 10⁶ cells. Summarized data for oxygen consumption capacity. , , , ^# vs. controls. (c, d) p-PDHE1α and total PDHE1α (t-PDHE1α) protein expression in human fibroblasts treated with TGFβ1 alone (10 ng/mL) or with TGFβ1 and Pyr3 (10 μmol/L) for 24 h. , ^# vs. controls. (e, f) p-PDHE1α and t-PDHE1α protein expression in dermal fibroblasts from WT or Trpc3^-/- mice. vs. WT. (g) DHE staining for cellular ROS, (h) mitochondrial ROS levels, (i) H₂O₂ production, and (j) ATP levels in human fibroblasts under TGFβ1 treatment (10 ng/mL) or TGFβ1 and Pyr3 treatment (10 μmol/L) for 24 h. , , , ^# vs. controls.

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